Introduction
Rescue RNA from FFPE
Dual de-crosslinking and dual reverse transcription improve RNA recovery and coverage for archived FFPE samples.
Introduction
Highlights
Dual De-crosslinking Strategy
Improves RNA accessibility in FFPE samples
Efficient Capture of Degraded RNA
High recovery from heavily crosslinked material
TSO-Extended Reverse Transcription
Enables longer RNA sequence coverage
Expanded Priming Strategy
Oligo(dT) and random priming support diverse RNA species
Workflow
From Sample to Insight in Six Steps
Sample Prep

Sample Prep

Nuclei isolation & RNA QC
In-situ Reverse Transcription

In-situ Reverse Transcription

Catch RNA with Random Primer
Microfluidic Partitioning

Microfluidic Partitioning

Automated separation and labeling cells and RNA
Library Prep

Library Prep

Construct expression libraries
Sequencing

Sequencing

Generate datasets
Bioinformatic Analysis

Bioinformatic Analysis

Data processing and visualization
Product Features
Sample Types
Components
Product Name
Product Code
Specification
SeekOne™ DD FFPE Single Cell Transcriptome-seq Kit
K02101-08
8 tests
Components Name
Components Code
Specification
SeekOne™ DD Chip S3 Kit
K00202-0801
8 tests
SeekOne™ DD scFAST-seq Barcoded Beads Kit
K00801-0802
8 tests
SeekOne™ DD scFAST-seq Reverse Transcription Kit
K00801-0803
8 tests
SeekOne™ DD Single Cell Barcode Ligation Kit
K02101-0804
8 tests
SeekOne™ DD Single Cell Decrosslinking Kit
K02101-0806
8 tests
SeekOne™ DD Single Cell Library Amplification Kit
K02101-0807
8 tests
SeekOne™ DD Single Cell Cleanup Kit
K00202-0805
8 tests
Compatible Instrument
SeekOne™ Digital Droplet System
A high-throughput, automated single-cell multi-omics platform based on microfluidic water-in-oil technology for single cell/nucleus separation and barcoding.
Data Analysis

SeekSoul™ Tools

Comprehensive software suite for processing SeekOne™ single-cell transcriptome data, covering RNA, full-length, FFPE, and immune repertoire analyses.

SeekSoul™ Online

Cloud-based platform for single-cell multi-omics data analysis and visualization — zero code, full workflow, accessible anywhere.
资源 25
Demo Datasets
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Publications
[ British Journal of Cancer ]

Predictive factors for neoadjuvant combined immunotherapy in gastric adenocarcinoma: Focusing on the primitive enterocyte phenotype and PVR

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